پروتکل استخراج DNA از باله ی ماهی
DNA Extraction from fish fins
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Put 1cm2 of fin tissue to 700ml of TNES-Urea Buffer
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Add 3.5 ml of 10mg/ml Proteinase-K
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Digest O/N at 37 C
· Wear gloves and work in fume hood when working with Phenol and Chloroform
· Dispose of waste in Hazardous waste container
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Extract two times with an equal volume of Phenol:Chloroform:Isoamyl Alcohol (25:24:1)
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Add 700ml of P:C:I
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rotate or invert for 10 minutes
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Spin at full speed for 10 minutes (Centrifuge in hood)
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remove the top aqueous layer into new tube.
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repeat with an equal volume of P:C:I
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Extract one time with an equal volume of C:I (24:1)
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Add 1/10 volume of 3M NaOAc pH 5.3
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Add 2 volumes 100% EtOH
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Either (1) collect spool of DNA with a pipette and wash in a new tube of 70% EtOH or (2) spin for 10 min. at full speed, pour off supernatent and wash pellet in 70% EtOH
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Pour or aspirate off EtOH and let pellet air dry.
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Resuspend pellet in water or TE.
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با عرض معذرت از تاخیر بیش از حد ...![]()
اگر شخصی پروتکل کامل تری از استخراج داره ممنون میشم راهنماییم کنه ... ![]()






















